Rescue of the streptomycin antibiotic activity by using streptidine as a "decoy acceptor" for the aminoglycoside-inactivating enzyme adenyl transferase.
نویسندگان
چکیده
The use of streptidine as a "decoy acceptor" allows the antibiotic activity of streptomycin to recover against the Escherichia coli strain overexpressing the aminoglycoside-modifying enzyme 6-O-adenyl transferase.
منابع مشابه
Molecular recognition of aminoglycoside antibiotics by bacterial defence proteins: NMR study of the structural and conformational features of streptomycin inactivation by Bacillus subtilis aminoglycoside-6-adenyl transferase.
The molecular recognition of streptomycin by Bacillus subtilis aminoglycoside-6-adenyl transferase has been analysed by a combination of NMR techniques and molecular dynamic simulations. This protein inactivates streptomycin by transferring an adenyl group to position six of the streptidine moiety. Our combined approach provides valuable information about the bioactive conformation for both the...
متن کاملAminoglycoside-inactivating enzymes in clinical isolates of Streptococcus faecalis. An explanation for resistance to antibiotic synergism.
Clinical isolates of enterococci (Streptococcus faecalis) with high-level resistance to both streptomycin and kanamycin (minimal inhibitory concentration >2,000 mug/ml), and resistant to synergism with penicillin and streptomycin or kanamycin were examined for aminoglycoside-inactivating enzymes. All of the 10 strains studied had streptomycin adenylyltransferase and neomycin phosphotransferase ...
متن کاملEnzymic Studies on the Biosynthesis of Streptomycin
1; Heat extracts of mature mycelia of streptomycin-producing strains of Sfreptomyces contain two compounds which, when incubated with arginine-guanidino-‘*C plus a crude enzyme preparation from S. griseus ATCC 12475 containing a&nine: X amidinotrausferase, give products detected as radioactive Peaks I and II. Heat extracts from S. bluensis var. bluensis, which synthesizes bluensomycin, a monogu...
متن کاملThe Jodrnal of Biological Chemxstry
1; Heat extracts of mature mycelia of streptomycin-producing strains of Sfreptomyces contain two compounds which, when incubated with arginine-guanidino-‘*C plus a crude enzyme preparation from S. griseus ATCC 12475 containing a&nine: X amidinotrausferase, give products detected as radioactive Peaks I and II. Heat extracts from S. bluensis var. bluensis, which synthesizes bluensomycin, a monogu...
متن کاملRiboswitch Control of Aminoglycoside Antibiotic Resistance
The majority of riboswitches are regulatory RNAs that regulate gene expression by binding small-molecule metabolites. Here we report the discovery of an aminoglycoside-binding riboswitch that is widely distributed among antibiotic-resistant bacterial pathogens. This riboswitch is present in the leader RNA of the resistance genes that encode the aminoglycoside acetyl transferase (AAC) and aminog...
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عنوان ژورنال:
- Chemical communications
دوره 27 شماره
صفحات -
تاریخ انتشار 2007